Journal: Nature biotechnology

Article Title: Development and function of human innate immune cells in a humanized mouse model

doi: 10.1038/nbt.2858

Figure Lengend Snippet: a , Percentages of human myeloid cells (hCD33 + ) among human hematopoietic cells (hCD45 + ) in the blood of the indicated recipient mice, engrafted as newborns by intra-hepatic injection of fetal liver CD34 + cells after X-ray preconditioning. Each symbol represents an individual mouse and the red bars indicate mean values (n=20-113; statistical analysis is shown in ). b , Composition of human white blood cells in the same mice as in a (n=20-113 mice/group; n=8 human donors; error bars indicate SEM). c , Immunohistological staining of human myeloid cells (hCD68 + ) in non-lymphoid tissues of the indicated recipient mice. The black bar represents 20 μm, and the images shown are representative of at least three mice analyzed per group. d-e , Representative flow cytometry analysis ( d ) and frequencies ( e ) of human monocyte subsets, identified by expression of CD14 and CD16 among hCD45 + CD33 + cells in the blood of recipient mice (n=8-12 mice/group; error bars indicate SEM). Dot plots in d are gated on CD33 hi SSC lo cells to show the subset distribution among monocytic cells. f , Human monocytes in the blood of MISTRG recipients and human monocytes from a human donor were stained with the indicated antibodies. Staining with isotype control antibodies is shown in red and specific antibodies in blue.

Article Snippet: Human CD33 + cells were enriched by magnetic isolation (EasySep CD33 selection kit, StemCell Technologies).

Techniques: Injection, Staining, Flow Cytometry, Expressing, Control